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| The candidate tumour suppressor p33(ING1) cooperates with p53 in cell growth control |
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| Author(s): Garkavtsev I, Grigorian IA, Ossovskaya VS, Chernov MV, Chumakov PM, Gudkov AV |
| Source: NATURE Volume: 391 Issue: 6664 Pages: 295-298 Published: JAN 15 1998 |
| Times Cited: 204 References: 18 |
| Abstract: The candidate tumour-suppressor gene ING1 has been identified by using the genetic suppressor element (GSE) methodology(1). ING1 encodes a nuclear protein, p33(ING1), overexpression of which inhibits growth of different cell lines. The properties of p33(ING1) suggest its involvement in the negative regulation of cell proliferation and in the control of cellular ageing, anchorage dependence and apoptosis(1-3). These cellular functions depend largely on the activity of p53, a tumour-suppressor gene that determines the cellular response to various types of stress(4). Here we report that the biological effects of ING1 and p53 are interrelated and require the activity of both genes: neither of the two genes can, on its own, cause growth inhibition when the other one is suppressed. Furthermore, activation of transcription from the P21/WAF1 promoter, a key mechanism of p53-mediated growth control, depends on the expression of ING1. A physical association between p33(ING1) and p53 proteins has been detected by immunoprecipitation. These results indicate that p33(ING1) is a component of the p53 signalling pathway that cooperates with p53 in the negative regulation of cell proliferation by modulating p53-dependent transcriptional activation. |
| Document Type: Article |
| Language: English |
| Reprint Address: Gudkov, AV (reprint author), Univ Illinois, Coll Med, Dept Mol Genet, Chicago, IL 60607 USA |
Addresses:
1. Univ Illinois, Coll Med, Dept Mol Genet, Chicago, IL 60607 USA
2. Univ Calgary, Dept Med Biochem, Calgary, AB T2N 4N1 Canada
3. Univ Calgary, So Alberta Canc Res Ctr, Calgary, AB T2N 4N1 Canada
4. VA Engelhardt Mol Biol Inst, Moscow 117984, Russia
5. Cleveland Clin Fdn, Cleveland, OH 44195 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: YR328 |
| ISSN: 0028-0836 |
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