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| Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants |
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| Author(s): Nicolson T, Rusch A, Friedrich RW, Granato M, Ruppersberg JP, Nusslein-Volhard C |
| Source: NEURON Volume: 20 Issue: 2 Pages: 271-283 Published: FEB 1998 |
| Times Cited: 93 References: 48 |
| Abstract: The molecular basis of sensory hair cell mechanotransduction is largely unknown. In order to identify genes that are essential for mechanosensory hair cell function, we characterized a group of recently isolated zebrafish motility mutants. These mutants are defective in balance and swim in circles but have no obvious morphological defects. We examined the mutants using calcium imaging of acoustic-vibrational and tactile escape responses, high resolution microscopy of sensory neuroepithelia in live larvae, and recordings of extracellular hair cell potentials (microphonics). Based on the analyses, we have identified several classes of genes. Mutations in sputnik and mariner affect hair bundle integrity. Mutant astronaut and cosmonaut hair cells have relatively normal microphonics and thus appear to affect events downstream of mechanotransduction. Mutant orbiter, mercury, and gemini larvae have normal hair cell morphology and yet do not respond to acoustic-vibrational stimuli. The microphonics of lateral line hair cells of orbiter, mercury, and gemini larvae are absent or strongly reduced. Therefore, these genes may encode components of the transduction apparatus. |
| Document Type: Article |
| Language: English |
| Reprint Address: Nicolson, T (reprint author), Max Planck Inst Entwicklungsbiol, Genet Abt, Spemannstr 35, D-72076 Tubingen, Germany |
Addresses:
1. Max Planck Inst Entwicklungsbiol, Genet Abt, D-72076 Tubingen, Germany
2. Max Planck Inst Entwicklungsbiol, Phys Biol Abt, D-72076 Tubingen, Germany
3. Max Planck Inst Entwicklungsbiol, Inst Physiol, D-72076 Tubingen, Germany
4. Univ Tubingen, Hals Nasen Ohren Klin, Sekt Sensor Biophys, D-72076 Tubingen, Germany
5. Univ Penn, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 USA |
| Subject Category: Neurosciences |
| IDS Number: YY510 |
| ISSN: 0896-6273 |
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