| | |  | | | | Record from Web of Science® | | | | | | |
| Efficacy of a food plant-based oral cholera toxin B subunit vaccine |
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| Author(s): Arakawa T, Chong DKX, Langridge WHR |
| Source: NATURE BIOTECHNOLOGY Volume: 16 Issue: 3 Pages: 292-297 Published: MAR 1998 |
| Times Cited: 170 References: 36 |
| Abstract: Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G(M1)-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G(M1)-ganglioside, These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin. |
| Document Type: Article |
| Language: English |
| Reprint Address: Langridge, WHR (reprint author), Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350 USA |
Addresses:
1. Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350 USA
2. Loma Linda Univ, Sch Med, Ctr Mol Biol & Gene Therapy, Loma Linda, CA 92350 USA
3. Loma Linda Univ, Sch Med, Dept Biochem, Loma Linda, CA 92350 USA |
| Publisher: NATURE AMERICA INC, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: ZA515 |
| ISSN: 1087-0156 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |