| Base-calling of automated sequencer traces using phred. II. Error probabilities |
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| Author(s): Ewing B, Green P |
| Source: GENOME RESEARCH Volume: 8 Issue: 3 Pages: 186-194 Published: MAR 1998 |
| Times Cited: 1,978 References: 6 |
| Abstract: Elimination of the data processing bottleneck in high-throughput sequencing will require both improved accuracy of data processing software and reliable measures of that accuracy. We have developed and implemented in our base-calling program phred the ability to estimate a probability of error for each base-call, as a function of certain parameters computed from the trace data. These error probabilities are shown here to be valid (correspond to actual error rates] and to have high power to discriminate correct base-calls from incorrect ones, For read data collected under several different chemistries and electrophoretic conditions. They play a critical role in our assembly program phrap and our finishing program consed. |
| Document Type: Article |
| Language: English |
| Reprint Address: Green, P (reprint author), Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA |
Addresses:
1. Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: ZE853 |
| ISSN: 1054-9803 |