| | |  | | | | Record from Web of Science® | | | | | | |
| Linker histone tails and N-tails of histone H3 are redundant: Scanning force microscopy studies of reconstituted fibers |
|
|
| Author(s): Leuba SH, Bustamante C, van Holde K, Zlatanova J |
| Source: BIOPHYSICAL JOURNAL Volume: 74 Issue: 6 Pages: 2830-2839 Published: JUN 1998 |
| Times Cited: 51 References: 34 |
| Abstract: The mechanisms responsible for organizing linear arrays of nucleosomes into the three-dimensional structure of chromatin are still largely unknown. In a companion paper (Leuba, S. H., et at. 1998. Biophys. J. 74:2823-2829), we study the contributions of linker histone domains and the N-terminal tail of core histone H3 to extended chromatin fiber structure by scanning force microscopy imaging of mildly trypsinized fibers. Here we complement and extend these studies by scanning force microscopy imaging of selectively reconstituted chromatin fibers, which differ in subtle but distinctive ways in their histone composition. We demonstrate an absolute requirement for the globular domain of the linker histones and a structural redundancy of the tails of linker histones and of histone H3 in determining conformational stability. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zlatanova, J (reprint author), Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA |
Addresses:
1. Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA
2. Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA
3. Univ Oregon, Dept Chem, Eugene, OR 97403 USA
4. Univ Oregon, Howard Hughes Med Inst, Eugene, OR 97403 USA
5. Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria |
| Publisher: BIOPHYSICAL SOCIETY, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA |
| Subject Category: Biophysics |
| IDS Number: ZT214 |
| ISSN: 0006-3495 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |