| | |  | | | | Record from Web of Science® | | | | | | |
| Functional reconstitution of a prokaryotic K+ channel |
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| Author(s): Heginbotham L, Kolmakova-Partensky L, Miller C |
| Source: JOURNAL OF GENERAL PHYSIOLOGY Volume: 111 Issue: 6 Pages: 741-749 Published: JUN 1998 |
| Times Cited: 82 References: 33 |
| Abstract: SliK, a K+ channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative Rb-86(+) flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K+ over Na+ and is inhibited by micromolar concentrations of Ba2+, mirroring the basic permeation characteristic of eukaryotic K+ channels studied by electrophysiological methods. Rb-86(+) uptake kinetics and equilibrium measurements also demonstrate that the purified protein is fully active. |
| Document Type: Article |
| Language: English |
| Reprint Address: Miller, C (reprint author), Brandeis Univ, Howard Hughes Med Inst, Dept Biochem, 415 South St,HHMI, Waltham, MA 02254 USA |
Addresses:
1. Brandeis Univ, Howard Hughes Med Inst, Dept Biochem, Waltham, MA 02254 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Physiology |
| IDS Number: ZT852 |
| ISSN: 0022-1295 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |