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| Immunohistochemical study of colorectal tumors for expression of a novel transmembrane carbonic anhydrase, MN/CA IX, with potential value as a marker of cell proliferation |
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| Author(s): Saarnio J, Parkkila S, Parkkila AK, Haukipuro K, Pastorekova S, Pastorek J, Kairaluoma MI, Karttunen TJ |
| Source: AMERICAN JOURNAL OF PATHOLOGY Volume: 153 Issue: 1 Pages: 279-285 Published: JUL 1998 |
| Times Cited: 107 References: 23 |
| Abstract: Carbonic anhydrase isoenzyme IX, MN/CA IX, is a recently discovered member of the carbonic anhydrase (CA) gene family with a suggested function in acid-base balance, intercellular communication, and cell proliferation, Increased expression of MN/CA IX has been observed with certain epithelial tumors, We investigated the expression of MN/CA IX in 69 colorectal neoplasms, consisting of 1 juvenile polyp, 8 hyperplastic polyps, 39 adenomatous lesions, 21 carcinomas, and 7 metastases. Tissue sections were immunostained with a monoclonal antibody specific to MN/CA IX, The proliferative activity of the tumor cells was evaluated by Ki-67 antigen immunoreactivity, The hyperplastic polyps showed a weak or moderate reaction for MN/CA IX only in the cryptal epithelium, as did the normal intestinal mucosa, The adenomas showed immunoreactivity mainly in the superficial part of the mucosa, whereas the distribution in the carcinomas and metastases was more diffuse. Comparative immunostaining of serial sections for Ki-67, a well established marker of cell proliferation, confirmed that MN/CA IX is expressed in areas with high proliferative capacity. Our results show abnormal MN/CA IX expression in colorectal neoplasms, suggesting its involvement in their pathogenesis. The co-occurrence of MN/CA IX and Ki-GSI in the same tumor cells indicates its potential for use as a marker of increased proliferation in the colorectal mucosa. |
| Document Type: Article |
| Language: English |
| Reprint Address: Saarnio, J (reprint author), Univ Oulu, Dept Surg, Kajaanintie 52 A, SF-90220 Oulu, Finland |
Addresses:
1. Univ Oulu, Dept Surg, SF-90220 Oulu, Finland
2. Univ Oulu, Dept Anat, SF-90220 Oulu, Finland
3. Univ Oulu, Dept Pathol, SF-90220 Oulu, Finland
4. Slovak Acad Sci, Inst Virol, Bratislava, Slovakia |
| Publisher: AMER SOC INVESTIGATIVE PATHOLOGY, INC, 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 USA |
| Subject Category: Pathology |
| IDS Number: ZX920 |
| ISSN: 0002-9440 |
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