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| Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint |
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| Author(s): Sun ZX, Hsiao J, Fay DS, Stern DF |
| Source: SCIENCE Volume: 281 Issue: 5374 Pages: 272-274 Published: JUL 10 1998 |
| Times Cited: 244 References: 30 |
| Abstract: The Rad53 protein kinase of Saccharomyces cerevisiae is required for checkpoints that prevent cell division in cells with damaged or incompletely replicated DNA, The Rad9 protein was phosphorylated in response to DNA damage, and phosphorylated Rad9 interacted with the COOH-terminal forkhead homology-associated (FHA) domain of Rad53, Inactivation of this domain abolished DNA damage-dependent Rad53 phosphorylation, G(2)/M cell cycle phase arrest, and increase of RNR3 transcription but did not affect replication inhibition-dependent Rad53 phosphorylation, Thus, Rad53 integrates DNA damage signals by coupling with phosphorylated Rad9, The hitherto uncharacterized FHA domain appears to be a modular protein-binding domain. |
| Document Type: Article |
| Language: English |
| Reprint Address: Stern, DF (reprint author), Yale Univ, Sch Med, Dept Pathol, 310 Cedar St,Room BML 342, New Haven, CT 06520 USA |
Addresses:
1. Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06520 USA
2. Yale Univ, Dept Biol, New Haven, CT 06511 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: ZZ286 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |