| | |  | | | | Record from Web of Science® | | | | | | |
| Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei |
|
|
| Author(s): Wakayama T, Perry ACF, Zuccotti M, Johnson KR, Yanagimachi R |
| Source: NATURE Volume: 394 Issue: 6691 Pages: 369-374 Published: JUL 23 1998 |
| Times Cited: 1,064 References: 26 |
| Abstract: Until recently, fertilization was the only way to produce viable mammalian offspring, a process implicitly involving male and female gametes, However, techniques involving fusion of embryonic or fetal somatic cells with enucleated oocytes have become steadily more successful in generating cloned young(1-3). Dolly the sheep(4) was produced by electrofusion of sheep mammary-derived cells with enucleated sheep oocytes. Here we investigate the factors governing embryonic development by introducing nuclei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mouse oocytes. We found that some enucleated oocytes receiving Sertoli or neuronal nuclei developed in vitro and implanted following transfer, but none developed beyond 8.5 days post coitum; however, a high percentage of enucleated oocytes receiving cumulus nuclei developed in vitro. Once transferred, many of these embryos implanted and, although most were subsequently resorbed, a significant proportion (2 to 2.8%) developed to term. These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development. |
| Document Type: Article |
| Language: English |
| Reprint Address: Yanagimachi, R (reprint author), Univ Hawaii, John A Burns Sch Med, Dept Anat & Reprod Biol, Honolulu, HI 96822 USA |
Addresses:
1. Univ Hawaii, John A Burns Sch Med, Dept Anat & Reprod Biol, Honolulu, HI 96822 USA
2. Univ Tokyo, Fac Agr, Dept Vet Anat, Bunkyo Ku, Tokyo 113, Japan
3. Babraham Inst, Dept Signalling, Cambridge CB2 4AT, England
4. Univ Pavia, Dipartimento Biol Anim, Lab Biol Sviluppo, I-27100 Pavia, Italy
5. Jackson Lab, Bar Harbor, ME 04609 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 103QF |
| ISSN: 0028-0836 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |