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| Effect of arbutin on melanogenic proteins in human melanocytes |
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| Author(s): Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M |
| Source: PIGMENT CELL RESEARCH Volume: 11 Issue: 4 Pages: 206-212 Published: AUG 1998 |
| Times Cited: 33 References: 42 |
| Abstract: The inhibitory effect of arbutin, a naturally occurring beta-D-glucopyranoside derivative of hydroquinone, on melanogenesis was studied biochemically by using human melanocytes in culture. Cells were cultured in the presence of different concentrations of arbutin. The maximum concentration of arbutin that was not inhibitory to growth of the cells was 100 mu g/ml. At that concentration, melanin synthesis was inhibited significantly by similar to 20% after 5 days, compared with untreated cells. This phenotypic change was associated with the inhibition of tyrosinase and DHICA polymerase activities, and the degree of inhibition was dose dependent. No significant difference in DOPAchrome tautomerase (DT) activity was observed before or after arbutin treatment. Western blotting experiments revealed there were no changes in protein content or in molecular size of tyrosinase, TRP-1 or TRP-2, indicating that inhibition of tyrosinase activity by arbutin might be due to effects at the post-translational level. |
| Document Type: Article |
| Language: English |
| Reprint Address: Funasaka, Y (reprint author), Kobe Univ, Sch Med, Dept Dermatol, Chuo Ku, 7-5-1 Kusonoki Cho, Kobe, Hyogo 6500017 Japan |
Addresses:
1. Kobe Univ, Sch Med, Dept Dermatol, Chuo Ku, Kobe, Hyogo 6500017 Japan
2. Yale Univ, Sch Med, Dept Dermatol, New Haven, CT 06510 USA |
| Publisher: MUNKSGAARD INT PUBL LTD, 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK |
| Subject Category: Cell Biology; Dermatology |
| IDS Number: 110KJ |
| ISSN: 0893-5785 |
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