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| A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae |
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| Author(s): Jaspersen SL, Charles JF, Tinker-Kulberg RL, Morgan DO |
| Source: MOLECULAR BIOLOGY OF THE CELL Volume: 9 Issue: 10 Pages: 2803-2817 Published: OCT 1998 |
| Times Cited: 174 References: 88 |
| Abstract: Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinase-cyclin complexes, primarily by ubiquitin-dependent cyclin proteolysis. Cyclin destruction is regulated by a ubiquitin ligase known as the anaphase-promoting complex (APC). In the budding yeast Saccharomyces cerevisiae, members of a large class of late mitotic mutants, including cdc15, cdc5, cdc14, dbf2, and tem1, arrest in anaphase with a phenotype similar to that of cells expressing nondegradable forms of mitotic cyclins. We addressed the possibility that the products of these genes are components of a regulatory network that governs cyclin proteolysis. We identified a complex array of genetic interactions among these mutants and found that the growth defect in most of the mutants is suppressed by overexpression of SPO12, YAK1, and SIC1 and is exacerbated by overproduction of the mitotic cyclin Clb2. When arrested in late mitosis, the mutants exhibit a defect in cyclin-specific APC activity that is accompanied by high Clb2 levels and low levels of the anaphase inhibitor Pds1. Mutant cells arrested in G1 contain normal APC activity. We conclude that Cdc15, Cdc5, Cdc14, Dbf2 and Tem1 cooperate in the activation of the APC in late mitosis but are not required for maintenance of that activity in G1. |
| Document Type: Article |
| Language: English |
| Reprint Address: Morgan, DO (reprint author), Univ Calif San Francisco, Dept Physiol, Box 0444, San Francisco, CA 94143 USA |
Addresses:
1. Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA |
| Publisher: AMER SOC CELL BIOLOGY, PUBL OFFICE, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Cell Biology |
| IDS Number: 128DC |
| ISSN: 1059-1524 |
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