| | |  | | | | Record from Web of Science® | | | | | | |
| Genomic organization and partial duplication of the human alpha 7 neuronal nicotinic acetylcholine receptor gene (CHRNA7) |
|
|
| Author(s): Gault J, Robinson M, Berger R, Drebing C, Logel J, Hopkins J, Moore T, Jacobs S, Meriwether J, Choi MJ, Kim EJ, Walton K, Buiting K, Davis A, Breese C, Freedman R, Leonard S |
| Source: GENOMICS Volume: 52 Issue: 2 Pages: 173-185 Published: SEP 1 1998 |
| Times Cited: 98 References: 75 |
| Abstract: The human alpha 7 neuronal nicotinic acetylcholine receptor gene (HGMW-approved symbol CHRNA7) has been characterized from genomic clones. The gene is similar in structure to the chick alpha 7 gene with 10 exons and conserved splice junction positions. The size of the human gene is estimated to be larger than 75 kb. A putative promoter 5' of the translation start in exon 1 has been cloned and sequenced. The promoter region lacks a TATA box and has a high GC content (77%). Consensus Sp1, AP-2, Egr-1, and CREB transcription factor binding sites appear to be conserved between bovine and human genes. The alpha 7 nAChR gene was found to be partially duplicated, with both loci mapping to the chromosome 15q13 region. A yeast artificial chromosome contig was constructed over a genetic distance of 5 cM that includes both alpha 7 loci and the region between them. Four novel exons are described, located in genomic clones containing the partially duplicated gene. The duplicated sequences, including the novel exons, are expressed in human brain. (C) 1998 Academic Press. |
| Document Type: Article |
| Language: English |
| Reprint Address: Leonard, S (reprint author), Univ Colorado, Hlth Sci Ctr, Dept Psychiat, Box C-268-71,4200 E 9th Ave, Denver, CO 80262 USA |
Addresses:
1. Univ Colorado, Hlth Sci Ctr, Dept Psychiat, Denver, CO 80262 USA
2. Univ Colorado, Hlth Sci Ctr, Dept Pharmacol, Denver, CO 80262 USA
3. Vet Affairs Med Ctr, Denver, CO 80262 USA
4. Univ Klinikum Essen, Essen, Germany |
| Publisher: ACADEMIC PRESS INC, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| Subject Category: Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: 128TD |
| ISSN: 0888-7543 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |