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| Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization |
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| Author(s): Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B |
| Source: MOLECULAR BIOLOGY OF THE CELL Volume: 9 Issue: 12 Pages: 3273-3297 Published: DEC 1998 |
| Times Cited: 2,146 References: 81 |
| Abstract: We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: alpha factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu. |
| Document Type: Article |
| Language: English |
| Reprint Address: Botstein, D (reprint author), Stanford Univ, Med Ctr, Dept Genet, Stanford, CA 94305 USA |
Addresses:
1. Stanford Univ, Med Ctr, Dept Genet, Stanford, CA 94305 USA
2. Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
3. Stanford Univ, Med Ctr, Dept Biochem, Stanford, CA 94305 USA
4. Howard Hughes Med Inst, Stanford, CA 94305 USA |
| Publisher: AMER SOC CELL BIOLOGY, PUBL OFFICE, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Cell Biology |
| IDS Number: 145TZ |
| ISSN: 1059-1524 |
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