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| The complete nucleotide sequence of the human immunoglobulin heavy chain variable region locus |
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| Author(s): Matsuda F, Ishii K, Bourvagnet P, Kuma K, Hayashida H, Miyata T, Honjo T |
| Source: JOURNAL OF EXPERIMENTAL MEDICINE Volume: 188 Issue: 11 Pages: 2151-2162 Published: DEC 7 1998 |
| Times Cited: 163 References: 57 |
| Abstract: The complete nucleotide sequence of the 957-kb DNA of the human immunoglobulin heavy chain variable (V-H) legion locus was determined and 43 novel V-H segments were identified. The region contains 123 V-H segments classifiable into seven different families, of which 79 are pseudogenes. Of the 44 V-H segments with an open reading frame, 39 are expressed as heavy chain proteins and 1 as mRNA, while the remaining 4 are not found in immunoglobulin cDNAs. Combinatorial diversity of V-H region was calculated to be similar to 6,000. Conservation of the promoter and recombination signal sequences was observed to be higher in functional VH segments than in pseudogenes. Phylogenetic analysis of 114 V-H segments clearly showed clustering of the V-H segments of each family. However, an independent branch in the tree contained a single V-H, V4-44.1P, sharing similar levels of homology to human V-H families and to those of other vertebrates. Comparison between different copies of homologous units that appear repeatedly across the locus clearly demonstrates that dynamic DNA reorganization of the locus took place at least eight times between 133 and 10 million years ago. One nonimmunoglobulin gene of unknown function was identified in the intergenic region. |
| Document Type: Article |
| Language: English |
| Reprint Address: Honjo, T (reprint author), Kyoto Univ, Grad Sch Med, Dept Med Chem, Sakyo Ku, Kyoto 60601, Japan |
Addresses:
1. Kyoto Univ, Grad Sch Med, Dept Med Chem, Sakyo Ku, Kyoto 60601, Japan
2. CNRS, INSERM, F-34033 Montpellier, France
3. Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 60601, Japan
4. Nara Med Univ, Kashihara, Nara 634 Japan |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Immunology; Medicine, Research & Experimental |
| IDS Number: 147JA |
| ISSN: 0022-1007 |
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