| | |  | | | | Record from Web of Science® | | | | | | |
| High density synthetic oligonucleotide arrays |
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| Author(s): Lipshutz RJ, Fodor SPA, Gingeras TR, Lockhart DJ |
| Source: NATURE GENETICS Volume: 21 Pages: 20-24 Supplement: Suppl. S Published: JAN 1999 |
| Times Cited: 1,225 References: 32 |
| Abstract: Experimental genomics involves taking advantage of sequence information to investigate and understand the workings of genes, cells and organisms. We have developed an approach in which sequence information is used directly to design high-density, two-dimensional arrays of synthetic oligonucleotides. The GeneChip(R) probe arrays are made using spatially patterned, light-directed combinatorial chemical synthesis, and contain up to hundreds of thousands of different oligonucleotides on a small glass surface. The arrays have been designed and used for quantitative and highly parallel measurements of gene expression, to discover polymorphic loci and to detect the presence of thousands of alternative alleles. Here, we describe the fabrication of the arrays, their design and some specific: applications to high-throughput genetic and cellular analysis. |
| Document Type: Review |
| Language: English |
| Reprint Address: Lipshutz, RJ (reprint author), Affymetrix Inc, 3380 Cent Expressway, Santa Clara, CA 95051 USA |
Addresses:
1. Affymetrix Inc, Santa Clara, CA 95051 USA |
| Publisher: NATURE AMERICA INC, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA |
| Subject Category: Genetics & Heredity |
| IDS Number: 156NU |
| ISSN: 1061-4036 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |