| | |  | | | | Record from Web of Science® | | | | | | |
| Gene targeting in the silkworm by use of a baculovirus |
|
|
| Author(s): Yamao M, Katayama N, Nakazawa H, Yamakawa M, Hayashi Y, Hara S, Kamei K, Mori H |
| Source: GENES & DEVELOPMENT Volume: 13 Issue: 5 Pages: 511-516 Published: MAR 1 1999 |
| Times Cited: 52 References: 17 |
| Abstract: The Bombyx mori fibroin light (L)-chain gene was cloned and the green fluorescent protein (GFP) gene inserted into exon 7. The chimeric L-chain-GFP gene was used to replace the polyhedrin gene of Autographa californica nucleopolyhedrovirus (AcNPV). This recombinant virus was used to target the L-chain-GFP gene to the L-chain region of the silkworm genome, female moths were infected with the recombinant virus and then mated with normal male moths. Genomic DNA from their progenies was screened for the desired targeting event. This analysis showed that the chimeric gene had integrated into the L-chain gene on the genome by homologous recombination and was stably transmitted through generations. The chimeric gene was expressed in the posterior silk gland, and the gene product was spun into the cocoon layer. |
| Document Type: Article |
| Language: English |
| Reprint Address: Mori, H (reprint author), Kyoto Inst Technol, Dept Appl Biol, Sakyo Ku, Kyoto 6068585, Japan |
Addresses:
1. Kyoto Inst Technol, Dept Appl Biol, Sakyo Ku, Kyoto 6068585, Japan
2. Natl Inst Sericultural & Entomol Sci, Tsukuba, Ibaraki 3058634 Japan
3. Fdn Kinugasakai, Text Res Inst, Kyoto 6038326, Japan |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: 176QK |
| ISSN: 0890-9369 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |