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| Transcript analysis of 250 novel yeast genes from chromosome XIV |
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| Author(s): Planta RJ, Brown AJP, Cadahia JL, Cerdan ME, De Jonge M, Gent ME, Hayes A, Kolen CPAM, Lombardia LJ, Sefton M, Oliver SG, Thevelein J, Tournu H, Van Delft YJ, Verbart DJ, Winderickx J |
| Source: YEAST Volume: 15 Issue: 4 Pages: 329-350 Published: MAR 15 1999 |
| Times Cited: 28 References: 39 |
| Abstract: The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for 250 novel ORFs on chromosome XIV. All transcripts were quantified by Northern analysis under three quasi-steady-state conditions (exponential growth on rich fermentative, rich non-fermentative, and minimal fermentative media) and eight transient conditions (glucose derepression, glucose upshift, stationary phase, nitrogen starvation, osmo-stress, heat-shock, and two control conditions). Transcripts were detected for 82% of the 250 ORFs, and only one ORF did not yield a transcript of the expected length (YNL285w). Transcripts ranged from low (62%), moderate (16%) to high abundance (2%) relative to the ACI mRNA, The levels of 73% of the 206 chromosome XIV transcripts detected fluctuated in response to the transient states tested. However, only a small number responded strongly to the transients: eight ORFs were induced upon glucose upshift: five were repressed by glucose; six were induced in response to nitrogen starvation three were induced in stationary phase; five were induced by osmo-stress; four were induced by heat-shock. These data provide useful clues about the general function of these ORFs and add to our understanding of gene regulation on a genome-wide basis. Copyright (C) 1999 John Wiley & Sons, Ltd. |
| Document Type: Article |
| Language: English |
| Reprint Address: Brown, AJP (reprint author), Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Foresterhill, Aberdeen AB25 2ZD, Scotland |
Addresses:
1. Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Aberdeen AB25 2ZD, Scotland
2. Vrije Univ Amsterdam, Dept Biochem & Mol Biol, NL-1081 HV Amsterdam, Netherlands
3. Univ La Coruna, Fac Ciencias, Dept Biol Celular & Mol, E-15071 La Coruna, Spain
4. Katholieke Univ Leuven, Mol Cell Biol Lab, B-3001 Heverlee, Belgium
5. UMIST, Dept Biomol Sci, Manchester M60 1QD, Lancs England |
| Publisher: JOHN WILEY & SONS LTD, BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Microbiology; Mycology |
| IDS Number: 177MK |
| ISSN: 0749-503X |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |