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| UV-A-induced decrease in nuclear factor-kappa B activity in human keratinocytes |
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| Author(s): Djavaheri-Mergny M, Gras MP, Mergny JL, Dubertret L |
| Source: BIOCHEMICAL JOURNAL Volume: 338 Pages: 607-613 Part: Part 3 Published: MAR 15 1999 |
| Times Cited: 24 References: 43 |
| Abstract: Previous reports have demonstrated an increase in nuclear factor-kappa B (NF-kappa B) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UVA radiation (320-400 nm) on NF-kappa B are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-kappa B DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-kappa B inducers. Moreover, UV-A radiation induces a decrease in NF-kappa B-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding I kappa B alpha (I kappa B is the NF-kappa B inhibitor), which is closely associated with NF-KB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-kappa B DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-kappa B activity in mammalian cells, probably through degradation of NF-kappa B protein subunits. These findings suggest that UV-A could modulate the NF-kappa B-dependent gene expression. |
| Document Type: Article |
| Language: English |
| Reprint Address: Djavaheri-Mergny, M (reprint author), Inst Curie, INSERM, U365, Rech Sect Biol, 26 Rue Ulm, F-75005 Paris, France |
Addresses:
1. Hop St Louis, INSERM, U312, Dermatol Lab, F-75475 Paris, France
2. MNHN, UMR 8646,Lab Biophys, CNRS, INSERM,U201, F-75231 Paris 05, France |
| Publisher: PORTLAND PRESS, 59 PORTLAND PLACE, LONDON W1N 3AJ, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: 179BX |
| ISSN: 0264-6021 |
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