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| Rho GTPases control polarity, protrusion, and adhesion during cell movement |
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| Author(s): Nobes CD, Hall A |
| Source: JOURNAL OF CELL BIOLOGY Volume: 144 Issue: 6 Pages: 1235-1244 Published: MAR 22 1999 |
| Times Cited: 714 References: 66 |
| Abstract: Cell movement is essential during embryogenesis to establish tissue patterns and to drive morphogenetic pathways and in the adult for tissue repair and to direct cells to sites of infection. Animal cells move by crawling and the driving force is derived primarily from the coordinated assembly and disassembly of actin filaments. The small GTPases, Rho, Rac, and Cdc42, regulate the organization of actin filaments and we have analyzed their contributions to the movement of primary embryo fibroblasts in an in vitro wound healing assay. Rac is essential for the protrusion of lamellipodia and for forward movement. Cdc42 is required to maintain cell polarity, which includes the localization of lamellipodial activity to the leading edge and the reorientation of the Golgi apparatus in the direction of movement. Rho is required to maintain cell adhesion during movement, but stress fibers and focal adhesions are not required. Finally, Ras regulates focal adhesion and stress fiber turnover and this is essential for cell movement. We conclude that the signal transduction pathways controlled by the four small GTPases, Rho, Rac, Cdc42, and Ras, cooperate to promote cell movement. |
| Document Type: Article |
| Language: English |
| Reprint Address: Hall, A (reprint author), UCL, MRC, Mol Cell Biol Lab, CRC Oncogene & Signal Transduct Grp, Gower St, London WC1E 6BT, England |
Addresses:
1. UCL, MRC, Mol Cell Biol Lab, CRC Oncogene & Signal Transduct Grp, London WC1E 6BT, England
2. UCL, Dept Biochem, London WC1E 6BT, England |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: 181YK |
| ISSN: 0021-9525 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |