| | |  | | | | Record from Web of Science® | | | | | | |
| Fluorescence polarization in homogeneous nucleic acid analysis |
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| Author(s): Chen XN, Levine L, Kwok PY |
| Source: GENOME RESEARCH Volume: 9 Issue: 5 Pages: 492-498 Published: MAY 1999 |
| Times Cited: 267 References: 25 |
| Abstract: A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allele-specific dye-labeled dideoxyribonucleoside triphosphates and a commercially available modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template, increasing similar to 10-fold the molecular weight of the fluorophore. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number of samples. |
| Document Type: Article |
| Language: English |
| Reprint Address: Chen, XN (reprint author), Washington Univ, Sch Med, Div Dermatol, St Louis, MO 63110 USA |
Addresses:
1. Washington Univ, Sch Med, Div Dermatol, St Louis, MO 63110 USA
2. Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA
3. Monsanto Life Sci Co, St Louis, MO 63198 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: 197CW |
| ISSN: 1088-9051 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |