| | |  | | | | Record from Web of Science® | | | | | | |
| Generation of oligodendroglial progenitors from neural stem cells |
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| Author(s): Zhang SC, Lundberg C, Lipsitz D, O'Connor LT, Duncan ID |
| Source: JOURNAL OF NEUROCYTOLOGY Volume: 27 Issue: 7 Pages: 475-489 Published: JUL 1998 |
| Times Cited: 59 References: 49 |
| Abstract: To understand how the differentiation of stem cells to oligodendroglial progenitors is regulated, we established cultures of neural stem cells from neonatal rat striatum in the presence of epidermal growth factor (EGF) as free-floating neurospheres that were then exposed to an increasing amount of B104 cell-conditioned medium (B104CM). The resultant cells proliferated in response to B104CM but no longer to EGF. In vitro analysis and transplantation studies indicated that these cells were committed to the oligodendroglial lineage, and they were thus referred to as oligospheres. Further characterization of their expression of early markers, cell cycle, migration, and self-renewal suggests that they were pre-O2A progenitors. RT-PCR analysis indicated that the oligosphere cells expressed mRNAs of platelet-derived growth factor a receptor in addition to fibroblast growth factor receptor but not EGF receptor; the latter two receptor mRNAs were expressed by neurosphere cells. Thus, the progression of stem cells to oligodendroglial progenitors is likely induced by factors in B104CM. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zhang, SC (reprint author), Univ Wisconsin, Sch Vet Med, Dept Med Sci, 2015 Linden Dr W, Madison, WI 53706 USA |
Addresses:
1. Univ Wisconsin, Sch Vet Med, Dept Med Sci, Madison, WI 53706 USA |
| Publisher: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS |
| Subject Category: Cell Biology; Neurosciences |
| IDS Number: 197UQ |
| ISSN: 0300-4864 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |