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| Promotion of chondrocyte proliferation by versican mediated by G1 domain and EGF-like motifs |
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| Author(s): Zhang Y, Cao L, Kiani C, Yang BL, Hu W, Yang BB |
| Source: JOURNAL OF CELLULAR BIOCHEMISTRY Volume: 73 Issue: 4 Pages: 445-457 Published: JUN 15 1999 |
| Times Cited: 33 References: 39 |
| Abstract: We have previously demonstrated that versican stimulated NIH3T3 fibroblast proliferation. Since versican is expressed in cartilage, we investigated whether versican plays a role in chondrocyte proliferation. We developed a technique to stably express a recombinant versican mini-gene in chicken chondrocytes, and its effect on chondrocyte proliferation was analyzed by the increase in cell number. The effect of cell adhesion on cell proliferation was tested. Finally, the versican mini-gene was truncated to assess the role of ECF-like motifs in cell proliferation. Expression of the recombinant versican mini-gene stimulated chondrocyte proliferation. Antisense oligonucleotides complementary to versican inhibited chondrocyte proliferation. The G1 domain of versican stimulated chondrocyte proliferation by destabilizing chondrocyte adhesion. Furthermore, deletion of the two EGF-like motifs from the G3 domain also reduced the function of versican in stimulating cell proliferation. Versican enhances chondrocyte proliferation through a mechanism involving its G1 and G3 domains. This finding may have implications for our understanding of the pathogenesis of various joint diseases. (C) 1999 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: Yang, BB (reprint author), Sunnybrook & Womens Coll, Hlth Sci Ctr, 2075 Bayview Ave,Res Bldg, Toronto, ON M4N 3M5 Canada |
Addresses:
1. Sunnybrook & Womens Coll, Hlth Sci Ctr, Toronto, ON M4N 3M5 Canada
2. Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M4N 3M5 Canada |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 199YY |
| ISSN: 0730-2312 |
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