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| Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction |
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| Author(s): Lo YMD, Wong IHN, Zhang J, Tein MSC, Ng MHL, Hjelm NM |
| Source: CANCER RESEARCH Volume: 59 Issue: 16 Pages: 3899-3903 Published: AUG 15 1999 |
| Times Cited: 99 References: 21 |
| Abstract: We have developed a quantitative method for methylation analysis of the p16 gene based on real-time methylation-specific PCR (MSP). Realtime MSP is sensitive enough to detect down to 10 genome equivalents of the methylated p16 sequence. Application of real-time MSP to DNA from tumor-derived cell Lines revealed complete concordance with conventional MSP analysis. Quantitative data generated by real-time MSP were expressed as the methylation index, which was defined as the percentage of bisulfite-converted DNA that consisted of methylated target sequences. The methylation index was shown to be inversely correlated with p16 gene transcription during demethylation treatment of cell lines with 5-aza-2'-deoxycytidine. The application of real-time MSP to bone marrow aspirates from patients with multiple myeloma revealed complete concordance with conventional MSP analysis. Real-time quantitative MSP may have applications in elucidating diverse biological processes involving DNA methylation and mag become a valuable diagnostic tool for detecting tumor-associated epigenetic changes in cancer patients. |
| Document Type: Article |
| Language: English |
| Reprint Address: Lo, YMD (reprint author), Chinese Univ Hong Kong, Dept Chem Pathol, Prince Wales Hosp, Room 38023,1-F Clin Sci Bldg,30-32 Ngan Shing St, Shatin, New Territories Hong Kong |
Addresses:
1. Chinese Univ Hong Kong, Dept Chem Pathol, Prince Wales Hosp, Shatin, New Territories Hong Kong
2. Chinese Univ Hong Kong, Dept Anat & Cellular Pathol, Prince Wales Hosp, Shatin, New Territories Hong Kong |
| Publisher: AMER ASSOC CANCER RESEARCH, PO BOX 11806, BIRMINGHAM, AL 35202 USA |
| Subject Category: Oncology |
| IDS Number: 228KJ |
| ISSN: 0008-5472 |
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