| | |  | | | | Record from Web of Science® | | | | | | |
| In vivo protein transduction: Delivery of a biologically active protein into the mouse |
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| Author(s): Schwarze SR, Ho A, Vocero-Akbani A, Dowdy SF |
| Source: SCIENCE Volume: 285 Issue: 5433 Pages: 1569-1572 Published: SEP 3 1999 |
| Times Cited: 1,065 References: 22 |
| Abstract: Delivery of therapeutic proteins into tissues and across the blood-brain barrier is severely limited by the size and biochemical properties of the proteins. Here it is shown that intraperitoneal injection of the 120-kilodalton beta-galactosidase protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in-delivery of the biologically active fusion protein to all tissues in mice, including the brain. These results open new possibilities for direct delivery of proteins into patients in the context of protein therapy, as well as for epigenetic experimentation with model organisms. |
| Document Type: Article |
| Language: English |
| Reprint Address: Dowdy, SF (reprint author), Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA |
Addresses:
1. Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA
2. Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA
3. Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 232FD |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |