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| Functions of the exosome in rRNA, snoRNA and snRNA synthesis |
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| Author(s): Allmang C, Kufel J, Chanfreau G, Mitchell P, Petfalski E, Tollervey D |
| Source: EMBO JOURNAL Volume: 18 Issue: 19 Pages: 5399-5410 Published: OCT 1 1999 |
| Times Cited: 229 References: 47 |
| Abstract: The yeast nuclear exosome contains multiple 3'-->5' exoribonucleases, raising the question of why so many activities are present in the complex. All components are required during the 3' processing of the 5.8S rRNA, together with the putative RNA helicase Dob1p/R/Mtr4p. During this processing three distinct steps can be resolved, and hand-over between different exonucleases appears to occur at least twice. 3' processing of snoRNAs (small nucleolar RNAs) that are excised from polycistronic precursors or from mRNA introns is also a multi-step process that involves the exosome, with final trimming specifically dependent on the Rrp6p component. The spliceosomal U4 snRNA (small nuclear RNA) is synthesized from a 3' extended precursor that is cleaved by Rntlp at sites 135 and 169 nt downstream of the mature 3' end. This cleavage is followed by 3'-->5' processing of the pre-snRNA involving the exosome complex and Dob1p. The exosome, together with Rntlp, also participates in the 3' processing of the U1 and U5 snRNAs, We conclude that the exosome is involved in the processing of many RNA substrates and that different components can have distinct functions. |
| Document Type: Article |
| Language: English |
| Reprint Address: Tollervey, D (reprint author), Univ Edinburgh, Inst Cell & Mol Biol, Swann Bldg,Kings Bldg, Edinburgh EH9 3JR, Midlothian Scotland |
Addresses:
1. Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian Scotland
2. Inst Pasteur, GIM Biotechnol, F-75724 Paris 15, France |
| Publisher: OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 246AK |
| ISSN: 0261-4189 |
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