| | |  | | | | Record from Web of Science® | | | | | | |
| The stratum corneum chymotryptic enzyme that mediates shedding and desquamation of skin cells is highly overexpressed in ovarian tumor cells |
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| Author(s): Tanimoto H, Underwood LJ, Shigemasa K, Yan Y, Clarke J, Parmley TH, O'Brien TJ |
| Source: CANCER Volume: 86 Issue: 10 Pages: 2074-2082 Published: NOV 15 1999 |
| Times Cited: 97 References: 13 |
| Abstract: BAGKGROUND. Proteases play essential roles in the process of tumor invasion and metastasis. The serine protease stratum corneum chymotryptic enzyme (SCCE) has been purified from human stratum corneum and is known to contribute to the cell shedding process by catalyzing the degradation of intercellular cohesive structures at the skin surface. The presence of SCCE on the surface of tumor cells suggests it also may contribute to the process of tumor cell shedding, resulting in early metastasis of carcinoma. METHODS. Gene expression of SCCE was investigated in 44 ovarian tumors (12 low malignant potential tumors and 32 carcinomas) and 10 normal ovaries by quantitative polymerase chain reaction (PCR). The PCR product was labeled with P-32 and a phosphoimager was used to determine the relative expression of SCCE compared with an internal control B-tubulin. mRNA transcripts were studied by Northern blot hybridization and protein expression and localization was examined by Western blot analysis and immunohistochemistry.
RESULTS. mRNA expression levels of SCCE were elevated significantly in 66.7% of 12 low malignant potential tumors and 78.1% of 32 carcinomas. Furthermore, SCCE protein was abundant in tumor cells and tumor cell lines that overexpressed the mRNA transcript.
CONCLUSIONS. The results of the current study suggest that SCCE frequently is overexpressed in ovarian tumors and therefore may contribute to tumor cell growth, tumor spread, and the metastatic potential of ovarian tumor cells. Cancer 1999;86:2074-82. (C) 1999 American Cancer Society.
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| Document Type: Article |
| Language: English |
| Reprint Address: O'Brien, TJ (reprint author), Univ Arkansas Med Sci Hosp, Dept Obstet & Gynecol, 4301 W Markham,Slot 718, Little Rock, AR 72205 USA |
Addresses:
1. Univ Arkansas Med Sci Hosp, Dept Obstet & Gynecol, Little Rock, AR 72205 USA
2. Univ Arkansas Med Sci Hosp, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA
3. Hiroshima Univ, Sch Med, Dept Obstet & Gynecol, Hiroshima, Japan |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA |
| Subject Category: Oncology |
| IDS Number: 256KH |
| ISSN: 0008-543X |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |