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| Prion protein expression aids cellular uptake and veratridine-induced release of copper |
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| Author(s): Brown DR |
| Source: JOURNAL OF NEUROSCIENCE RESEARCH Volume: 58 Issue: 5 Pages: 717-725 Published: DEC 1 1999 |
| Times Cited: 74 References: 36 |
| Abstract: The function of the prion protein is unknown despite suggestions it binds copper. Radioactive copper (Cu-67) was used to demonstrate that histidine-dependent uptake of copper by cerebellar cells in culture is related to the level of PrPc expression. Copper is released by neurones at the synapse. Veratridine-induced release from synapses was proportional to the level of PrPc expression. Veratridine-induced release can be abolished only from PrPc expressing cells by pretreatment with phosphatidyl-specific phospholipase C, an enzyme that cleaves PrPc from the cell surface. These results suggest that PrPc aids cellular copper uptake and may have a function at the synapse related to release of copper during transmission. (C) 1999 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: Brown, DR (reprint author), Univ Cambridge, Dept Biochem, Tennis Court Rd, Cambridge CB2 1QW, England |
Addresses:
1. Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
2. Univ Cambridge, MRC, Cambridge Ctr Brain Repair, Cambridge CB2 1QW, England |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA |
| Subject Category: Neurosciences |
| IDS Number: 259BM |
| ISSN: 0360-4012 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |