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| The new kallikrein-like gene, KLK-L2 - Molecular characterization, mapping, tissue expression, and hormonal regulation |
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| Author(s): Yousef GM, Diamandis EP |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 274 Issue: 53 Pages: 37511-37516 Published: DEC 31 1999 |
| Times Cited: 71 References: 37 |
| Abstract: Since in rodents the kallikreins are represented by a large multi-gene family, the restriction of this family in humans to three genes is somewhat surprising. In an effort to identify new human kallikrein genes, we examined a genomic area of about 300 kilobases on chromosome 19q13.3-q13.4, a region that contains most of the currently known kallikreins. By using the positional candidate approach, we were able to identify a new gene named KLK-L2 (for kallikrein-like gene 2). Screening of human EST libraries allowed us to delineate the full genomic and cDNA structure of the new gene. KLK-L2 consists of 5 coding exons and 4 introns and has significant similarities to other members of the kallikrein multi-gene family. Homology studies suggest that the protein is likely secreted. KLK-L2 is expressed mainly in breast, brain, and testis and to a lesser extent in many other tissues. KLK-L2 is up-regulated by estrogens and progestins in the breast cancer cell line BT-474. |
| Document Type: Article |
| Language: English |
| Reprint Address: Diamandis, EP (reprint author), Mt Sinai Hosp, Dept Pathol & Lab Med, 600 Univ Ave, Toronto, ON M5G 1X5 Canada |
Addresses:
1. Mt Sinai Hosp, Dept Pathol & Lab Med, Toronto, ON M5G 1X5 Canada
2. Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5G 1L5 Canada |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: 270GW |
| ISSN: 0021-9258 |
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