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| Construction of a genetic toggle switch in Escherichia coli |
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| Author(s): Gardner TS, Cantor CR, Collins JJ |
| Source: NATURE Volume: 403 Issue: 6767 Pages: 339-342 Published: JAN 20 2000 |
| Times Cited: 755 References: 26 |
| Abstract: It has been proposed(1) that gene-regulatory circuits with virtually any desired property can be constructed from networks of simple regulatory elements. These properties, which include multistability and oscillations, have been found in specialized gene circuits such as the bacteriophage lambda switch(2) and the Cyanobacteria circadian oscillator(3), However, these behaviours have not been demonstrated in networks of non-specialized regulatory components. Here we present the construction of a genetic toggle switch-a synthetic, bistable gene-regulatory network-in Escherichia coli and provide a simple theory that predicts the conditions necessary for bistability, The toggle is constructed from any two repressible promoters arranged in a mutually inhibitory network. It is flipped between stable states using transient chemical or thermal induction and exhibits a nearly ideal switching threshold. As a practical device, the toggle switch forms a synthetic, addressable cellular memory unit and has implications for biotechnology, biocomputing and gene therapy. |
| Document Type: Article |
| Language: English |
| Reprint Address: Collins, JJ (reprint author), Boston Univ, Dept Biomed Engn, 44 Cummington St, Boston, MA 02215 USA |
Addresses:
1. Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
2. Boston Univ, Ctr BioDynam, Boston, MA 02215 USA
3. Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 276VU |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |