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| The complete sequence of a heterochromatic island from a higher eukaryote |
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| Author(s): McCombie WR, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Yordan C, Sehkon M, Murray J, Sheet P, Cordes M, Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Simorowski J, May B, Ma P, Preston R, Vil D, See LH, Shekher M, Matero A, Shah R, Swaby I, O'Shaughnessy A, Rodriguez M, Hoffman J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Wilson RK, Martienssen R |
| Source: CELL Volume: 100 Issue: 3 Pages: 377-386 Published: FEB 4 2000 |
| Times Cited: 88 References: 89 |
| Abstract: Heterochromatin, constitutively condensed chromosomal material, is widespread among eukaryotes but incompletely characterized at the nucleotide level. We have sequenced and analyzed 2.1 megabases (Mb) of Arabidopsis thaliana chromosome 4 that includes 0.5-0.7 Mb of isolated heterochromatin that resembles the chromosomal knobs described by Barbara McClintock in maize. This isolated region has a low density of expressed genes, low levels of recombination and a low incidence of genetrap insertion. Satellite repeats were absent, but tandem arrays of long repeats and many transposons were found. Methylation of these sequences was dependent on chromatin remodeling. Clustered repeats were associated with condensed chromosomal domains elsewhere. The complete sequence of a heterochromatic island provides an opportunity to study sequence determinants of chromosome condensation. |
| Document Type: Article |
| Language: English |
| Reprint Address: Martienssen, R (reprint author), Cold Spring Harbor Lab, Cold Spring Harbor Plant Biol Grp, POB 100, Cold Spring Harbor, NY 11724 USA |
Addresses:
1. Cold Spring Harbor Lab, Cold Spring Harbor Plant Biol Grp, Cold Spring Harbor, NY 11724 USA
2. Cold Spring Harbor Lab, Lita Annenberg Hazen Genome Ctr, Cold Spring Harbor, NY 11724 USA
3. Washington Univ, Sch Med, Genome Sequencing Ctr, St Louis, MO 63108 USA
4. Appl Biosyst Inc, Foster City, CA 94494 USA
5. Axys, La Jolla, CA 92037 USA
6. N Carolina State Univ, Dept Forestry, Raleigh, NC 27695 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 282ET |
| ISSN: 0092-8674 |
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