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| 17 beta-Estradiol and ICI-182780 regulate the hair follicle cycle in mice through an estrogen receptor-alpha pathway |
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| Author(s): Chanda S, Robinette CL, Couse JF, Smart RC |
| Source: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM Volume: 278 Issue: 2 Pages: E202-E210 Published: FEB 2000 |
| Times Cited: 46 References: 43 |
| Abstract: Estradiol (E-2) applied topically twice weekly to mouse skin at doses as low as 1 nmol inhibited hair growth by blocking the transition of the hair follicle from the resting phase (telogen) to the growth phase (anagen). In contrast, application of less than or equal to 10 nmol of other steroids produced limited inhibition. Topical treatment with the estrogen receptor (ER) antagonist ICI-182780 reversed the effects of E-2, and when applied alone, ICI-182780 caused a telogen-to-anagen transition. Both Eg and ICI-182780 were highly effective at their site of application but not at distant sites, indicating the direct rather than secondary systemic nature of their effects. Western analysis detected a 65-kDa ER-alpha immunoreactive dermal protein, and Northern analysis revealed the presence of a 6.7-kb ER-alpha mRNA. A ribonuclease protection assay confirmed the presence of ER-alpha transcripts but failed to detect ER-beta transcripts. These findings implicate a skin-specific ER-alpha pathway in the regulation of the hair follicle cycle. |
| Document Type: Article |
| Language: English |
| Reprint Address: Chanda, S (reprint author), N Carolina State Univ, Dept Toxicol, Coll Vet Med, Raleigh, NC 27695 USA |
Addresses:
1. N Carolina State Univ, Dept Toxicol, Coll Vet Med, Raleigh, NC 27695 USA
2. N Carolina State Univ, Dept Anat Physiol Sci & Radiol, Coll Vet Med, Raleigh, NC 27695 USA
3. NIEHS, Receptor Biol Sect, Reprod & Dev Toxicol Lab, NIH, Res Triangle Pk, NC 27709 USA |
| Publisher: AMER PHYSIOLOGICAL SOC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Endocrinology & Metabolism; Physiology |
| IDS Number: 283CM |
| ISSN: 0193-1849 |
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