| | |  | | | | Record from Web of Science® | | | | | | |
| Analysis of human skin emanations by gas chromatography/mass spectrometry. 2. Identification of volatile compounds that are candidate attractants for the yellow fever mosquito (Aedes aegypti) |
|
|
| Author(s): Bernier UR, Kline DL, Barnard DR, Schreck CE, Yost RA |
| Source: ANALYTICAL CHEMISTRY Volume: 72 Issue: 4 Pages: 747-756 Published: FEB 15 2000 |
| Times Cited: 64 References: 58 |
| Abstract: Volatile compounds emanated from human skin were studied by gas chromatography/mass spectrometry (GC/MS). The purpose of this study was to identify compounds that may be human-produced kairomones which are used for host location by the mosquito, Aedes aegypti (L.). The procedure used to collect volatiles was chosen because of prior knowledge that attractive substances can be transferred from skin to glass by handling. Laboratory bioassays have shown that the residuum on the glass remains attractive to mosquitoes until the compounds of importance evaporate. The sampling and analytical procedures modeled the above-cited process as closely as possible except that the evaporation of compounds from the glass surface was accomplished by thermal desorption from glass beads in a heated GC injection port. This made possible the solventless injection of volatiles onto the column. The compounds were cryofocused on the head of the column with liquid nitrogen prior to GC separation. A single stage of mass spectrometry on a triple quadrupole instrument was used for mass analysis. A combination of electron ionization and pulsed positive ion/negative ion chemical ionization modes on two different GC columns (one polar, one relatively nonpolar) was used to identify most of the 346 compound peaks detected by this technique. |
| Document Type: Article |
| Language: English |
| Reprint Address: Bernier, UR (reprint author), Univ Florida, Dept Chem, POB 117200,Chem Lab Bldg, Gainesville, FL 32611 USA |
Addresses:
1. Univ Florida, Dept Chem, Gainesville, FL 32611 USA
2. USDA, Agr Res Serv, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA |
| Publisher: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA |
| Subject Category: Chemistry, Analytical |
| IDS Number: 285HE |
| ISSN: 0003-2700 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |