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| X-ray structure of beta-carbonic anhydrase from the red alga, Porphyridium purpureum, reveals a novel catalytic site for CO2 hydration |
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| Author(s): Mitsuhashi S, Mizushima T, Yamashita E, Yamamoto M, Kumasaka T, Moriyama H, Ueki T, Miyachi S, Tsukihara T |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 275 Issue: 8 Pages: 5521-5526 Published: FEB 25 2000 |
| Times Cited: 73 References: 24 |
| Abstract: The carbonic anhydrases (CAs) fall into three evolutionarily distinct families designated alpha-, beta-, and gamma-CAs based on their primacy structure. beta-CAs are present in higher plants, algae, and prokaryotes, and are involved in inorganic carbon utilization, Here, we describe the novel x-ray structure of beta-CA from the red alga, Porphyridium purpureum, at 2.2-Angstrom resolution using intrinsic zinc multiwavelength anomalous diffraction phasing. The CA monomer is composed of two internally repeating structures, being folded as a pair of fundamentally equivalent motifs of an alpha/beta domain and three projecting alpha-helices. The motif is obviously distinct from that of either alpha- or gamma-CAs. This homodimeric CA appears like a tetramer with a pseudo 222 symmetry. The active site zinc is coordinated by a Cys-Asp-His-Cys tetrad that is strictly conserved among the beta-CAs, No water molecule is found in a zinc-liganding radius, indicating that the zinc-hydroxide mechanism in alpha-CAs, and possibly in gamma-CAs, is not directly applicable to the case in beta-CAs. Zinc coordination environments of the CAs provide an interesting example of the convergent evolution of distinct catalytic sites required for the same CO2 hydration reaction. |
| Document Type: Article |
| Language: English |
| Reprint Address: Mitsuhashi, S (reprint author), Kamaishi Labs, Marine Biotechnol Inst, Kamaishi, Iwate 0260001 Japan |
Addresses:
1. Kamaishi Labs, Marine Biotechnol Inst, Kamaishi, Iwate 0260001 Japan
2. Osaka Univ, Inst Prot Res, Suita, Osaka 5650871 Japan
3. RIKEN, Harima Inst, Sayo, Hyogo 6795143 Japan
4. Japan Synchrotron Radiat Res Inst, Sayo, Hyogo 6795198 Japan |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: 286XQ |
| ISSN: 0021-9258 |
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