| | |  | | | | Record from Web of Science® | | | | | | |
| T2Rs function as bitter taste receptors |
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| Author(s): Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng LX, Guo W, Zuker CS, Ryba NJP |
| Source: CELL Volume: 100 Issue: 6 Pages: 703-711 Published: MAR 17 2000 |
| Times Cited: 403 References: 41 |
| Abstract: Bitter taste perception provides animals with critical protection against ingestion of poisonous compounds. In the accompanying paper, we report the characterization of a large family of putative mammalian taste receptors (T2Rs). Here we use a heterologous expression system to show that specific T2Rs function as bitter taste receptors. A mouse T2R (mT2R-5) responds to the bitter tastant cycloheximide, and a human and a mouse receptor (hT2R-4 and mT2R-8) responded to denatonium and 6-n-propyl-2-thiouracil. Mice strains deficient in their ability to detect cycloheximide have amino acid substitutions in the mT2R-5 gene; these changes render the receptor significantly less responsive to cycloheximide. We also expressed mT2R-5 in insect cells and demonstrate specific tastant-dependent activation of gustducin, a G protein implicated in bitter signaling. Since a single taste receptor cell expresses a large repertoire of T2Rs, these findings provide a plausible explanation for the uniform bitter taste that is evoked by many structurally unrelated toxic compounds. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zuker, CS (reprint author), NIH, Natl Inst Dent & Craniofacial Res, Bldg 10, Bethesda, MD 20892 USA |
Addresses:
1. NIH, Natl Inst Dent & Craniofacial Res, Bethesda, MD 20892 USA
2. Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
3. Univ Calif San Diego, Dept Biol, Dept Neurosci, La Jolla, CA 92093 USA
4. Aurora Biosci, San Diego, CA 92121 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 295TK |
| ISSN: 0092-8674 |
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