| | |  | | | | Record from Web of Science® | | | | | | |
| Engineering stability in gene networks by autoregulation |
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| Author(s): Becskei A, Serrano L |
| Source: NATURE Volume: 405 Issue: 6786 Pages: 590-593 Published: JUN 1 2000 |
| Times Cited: 397 References: 24 |
| Abstract: The genetic and biochemical networks which underlie such things as homeostasis in metabolism and the developmental programs of living cells, must withstand considerable variations and random perturbations of biochemical parameters(1-3). These occur as transient changes in, for example, transcription, translation, and RNA and protein degradation. The intensity and duration of these perturbations differ between cells in a population(4). The unique state of cells, and thus the diversity in a population, is owing to the different environmental stimuli the individual cells experience and the inherent stochastic nature of biochemical processes (for example, refs 5 and 6). It has been proposed, but not demonstrated, that autoregulatory, negative feedback loops in gene circuits provide stability(7), thereby limiting the range over which the concentrations of net-work components fluctuate. Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback. |
| Document Type: Article |
| Language: English |
| Reprint Address: Becskei, A (reprint author), European Mol Biol Lab, Meyerhofstr 1, D-69012 Heidelberg, Germany |
Addresses:
1. European Mol Biol Lab, D-69012 Heidelberg, Germany |
| Publisher: MACMILLAN PUBLISHERS LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 321HF |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |