| | |  | | | | Record from Web of Science® | | | | | | |
| 5 '-> 3 ' exonuclease-based real-time PCR assays for detecting the t(14;18)(q32;21): A survey of 162 malignant lymphomas and reactive specimens |
|
|
| Author(s): Estalilla OC, Medeiros LJ, Manning JT, Luthra R |
| Source: MODERN PATHOLOGY Volume: 13 Issue: 6 Pages: 661-666 Published: JUN 2000 |
| Times Cited: 24 References: 20 |
| Abstract: We describe our experience using two real-time polymerase chain reaction (PCR) assays for detecting the t(14;18)(q32;q21) in a large series of non-Hodgkin's lymphomas (NHLs), These assays utilize the 5'-->3' exonuclease activity of Tag polymerase, which cleaves a probe labeled with a fluorescent reporter dye at its 5' end and a quencher dye at its 3' end during the extension phase of PCR In a previous study, Luthra and colleagues developed these real-time PCR assays for detecting the t(14;18) involving the major and minor breakpoint cluster regions of the bcl-2 gene and assessed a small number of NHLs, In this larger study, we analyzed 135 NHLs, 6 Hodgkin's disease, 10 reactive biopsy specimens, and 11 peripheral blood specimens. The NHL group included 46 of 70 (65.7%) follicular NHLs, 1 of 2 (50%) diffuse small cleaved cell NHLs, and 13 of 24 (54.2%) diffuse large B-cell NHLs with the t(14;18) detected by conventional PCR methods. There was excellent agreement between the realtime and conventional PCR assays with overall concordance in 160 of 162 (98.8%) specimens. For the NHLs, concordance was found in 134 of 135 (99.3%) specimens. Disagreement was observed in one case of follicular NHL in which the real-time PCR assay detected bcl-2 minor breakpoint cluster region/JH DNA fusion sequences and the conventional method was negative. The overall concordance for 10 benign biopsy specimens and 11 normal peripheral blood samples was 20 of 21 (95.2%). One lymph node biopsy specimen that showed reactive follicular hyperplasia was positive for the bcl-2 minor breakpoint cluster region/JH DNA fusion sequences detected by the real-time PCR assay but was negative by conventional PCR methods. This patient had no clinical evidence of NHL, We conclude that realtime PCR assays for detecting the t(14;18) are sensitive, specific, and more convenient than conventional PCR methods. |
| Document Type: Article |
| Language: English |
| Reprint Address: Medeiros, LJ (reprint author), Univ Texas, MD Anderson Canc Ctr, Div Pathol & Lab Med, 1515 Holcombe Blvd,Box 085, Houston, TX 77030 USA |
Addresses:
1. Univ Texas, MD Anderson Canc Ctr, Div Pathol & Lab Med, Houston, TX 77030 USA |
| Publisher: LIPPINCOTT WILLIAMS & WILKINS, 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA |
| Subject Category: Pathology |
| IDS Number: 327XY |
| ISSN: 0893-3952 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |