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| Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle |
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| Author(s): Agrawal RK, Spahn CMT, Penczek P, Grassucci RA, Nierhaus KH, Frank J |
| Source: JOURNAL OF CELL BIOLOGY Volume: 150 Issue: 3 Pages: 447-459 Published: AUG 7 2000 |
| Times Cited: 104 References: 61 |
| Abstract: Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-Angstrom resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible. |
| Document Type: Article |
| Language: English |
| Reprint Address: Frank, J (reprint author), Howard Hughes Med Inst Hlth Res Inc, Wadsworth Ctr, Empire State Plaza,POB 509, Albany, NY 12201 USA |
Addresses:
1. Howard Hughes Med Inst Hlth Res Inc, Wadsworth Ctr, Albany, NY 12201 USA
2. SUNY Albany, Dept Biomed Sci, Wadsworth Ctr, Albany, NY 12201 USA
3. Max Planck Inst Mol Genet, D-14195 Berlin, Germany |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: 343XM |
| ISSN: 0021-9525 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |