| | |  | | | | Record from Web of Science® | | | | | | |
| Introducing a null mutation in the mouse K6 alpha and K6 beta genes reveals their essential structural role in the oral mucosa |
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| Author(s): Wong P, Colucci-Guyon E, Takahashi K, Gu CH, Babinet C, Coulombe PA |
| Source: JOURNAL OF CELL BIOLOGY Volume: 150 Issue: 4 Pages: 921-928 Published: AUG 21 2000 |
| Times Cited: 54 References: 29 |
| Abstract: Mammalian genomes feature multiple genes encoding highly related keratin 6 (K6) isoforms,These type II keratins show a complex regulation with constitutive and inducible components in several stratified epithelia, including the oral mucosa and skin. Two functional genes, K6 alpha and K6 beta, exist in a head-to-tail tandem array in mouse genomes. We inactivated these two genes simultaneously via targeting and homologous recombination. K6 null mice are viable and initially indistinguishable from their littermates. Starting at two to three days after birth, they show a growth delay associated with reduced milk intake and the presence of white plaques in the posterior region of dorsal tongue and upper palate. These regions are subjected to greater mechanical stress during suckling. Morphological analyses implicate the filiform papillae as being particularly sensitive to trauma in K6 alpha/K6 beta null mice, and establish the complete absence of keratin filaments in their anterior compartment. All null mice die about a week after birth, These studies demonstrate an essential structural role for K6 isoforms in the oral mucosa, and implicate filiform papillae as being the major stress bearing structures in dorsal tongue epithelium. |
| Document Type: Article |
| Language: English |
| Reprint Address: Coulombe, PA (reprint author), Johns Hopkins Univ, Sch Med, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD 21205 USA |
Addresses:
1. Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA
2. Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA
3. Inst Pasteur, Unite Biol Dev, F-75724 Paris, France
4. Kyoto Univ, Dept Dermatol, Kyoto 6068507, Japan |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: 347WQ |
| ISSN: 0021-9525 |
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