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| Regulation of intra-Golgi membrane transport by calcium |
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| Author(s): Porat A, Elazar Z |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 275 Issue: 38 Pages: 29233-29237 Published: SEP 22 2000 |
| Times Cited: 38 References: 33 |
| Abstract: Calcium cations play a critical role in regulating vesicular transport between different intracellular membrane-bound compartments. The role of calcium in transport between the Golgi cisternae, however, remains unclear. Using a well characterized cell-free intra-Golgi transport assay, we now show that changes in free Ca2+ concentration in the physiological range regulate this transport process. The calcium-chelating agent 1,2-bis(2-aminophenoxy)ethane-N,N,N,N'-tetraacetic acid blocked transport with an IC50 of approximately 0.8 mM. The effect of 1,2-bis(2- aminophenoxy)ethane-N,N,N',N'-tetraacetic acid was reversible by addition of fresh cytosol and was irreversible when performed in the presence of a Ca2+ ionophore that depletes calcium from lumenal stores, We demonstrate here that intra-Golgi transport is stimulated by low Ca2+ concentrations (20-100 nM) but is inhibited by higher concentrations (above 100 nM). Further, we show that calmodulin antagonists specifically block intra-Golgi transport, implying a role for calmodulin in mediating the effect of calcium. Our results suggest that Ca2+ efflux from intracellular pools may play an essential role in regulating intra-Golgi transport. |
| Document Type: Article |
| Language: English |
| Reprint Address: Elazar, Z (reprint author), Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel |
Addresses:
1. Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: 356JH |
| ISSN: 0021-9258 |
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