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| Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division |
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| Author(s): Bellaiche Y, Gho M, Kaltschmidt JA, Brand AH, Schweisguth F |
| Source: NATURE CELL BIOLOGY Volume: 3 Issue: 1 Pages: 50-57 Published: JAN 2001 |
| Times Cited: 105 References: 31 |
| Abstract: Cell-fate diversity is generated in part by the unequal segregation of cell-fate determinants during asymmetric cell divisions. In the Drosophila pupa, the pI sense organ precursor cell is polarized along the anterior-posterior axis of the fly and divides asymmetrically to generate a posterior pIIa cell and an anterior pIIb cell. The anterior pIIb cell specifically inherits the determinant Numb and the adaptor protein Partner of Numb (Pon). By labelling both the Pon crescent and the microtubules in living pupae, we show that determinants localize at the anterior cortex before mitotic-spindle formation; and that the spindle forms with random orientation and rotates to line up with the Pon crescent. By imaging living frizzled (fz) mutant pupae we show that Fz regulates the orientation of the polarity axis of pI, the initiation of spindle rotation and the unequal partitioning of determinants. We conclude that Fz participates in establishing the polarity of pI. |
| Document Type: Article |
| Language: English |
| Reprint Address: Schweisguth, F (reprint author), Ecole Normale Super, UMR 8544, 46 Rue Ulm, F-75230 Paris 05, France |
Addresses:
1. Ecole Normale Super, UMR 8544, F-75230 Paris 05, France
2. Univ Cambridge, Wellcome, CRC Inst, Cambridge CB2 1QR, England
3. Univ Cambridge, Dept Genet, Cambridge CB2 1QR, England |
| Publisher: MACMILLAN PUBLISHERS LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Cell Biology |
| IDS Number: 387XD |
| ISSN: 1465-7392 |
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