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| Increased expression of epidermal growth factor receptor in rat pleural mesothelial cells correlates with carcinogenicity of mineral fibres |
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| Author(s): Faux SP, Houghton CE, Hubbard A, Patrick G |
| Source: CARCINOGENESIS Volume: 21 Issue: 12 Pages: 2275-2280 Published: DEC 2000 |
| Times Cited: 23 References: 41 |
| Abstract: Asbestos fibres have been shown to stimulate the mitogen-activated protein kinase signalling cascade in rat pleural mesothelial (RPM) cells after autophosphorylation of the epidermal growth factor receptor (EGFR), We examined if mineral fibres with known carcinogenicity can be discriminated from materials with less or no carcinogenicity by their ability to up-regulate expression of EGFR protein in RPM cells in vitro. Crocidolite and erionite, two fibrous preparations with marked potential to induce mesothelioma, were associated with increases in EGFR protein expression over sham controls, whereas chrysotile asbestos and milled (non-fibrous) crocidolite did not. Intense patterns of EGFR protein expression were linked to RPM cells phagocytosing long fibres, To determine the role of EGFR expression in these cells, we assessed cell proliferation using an antibody against proliferating cell nuclear antigen (PCNA) in combination with an antibody against EGFR, In these co-localization studies, cells showed intense staining for EGFR protein 24 h before being PCNA positive at 48 h, These results suggest that carcinogenic fibres induce EGFR and initiate cell signalling cascades in mesothelial cells, leading to cell proliferation and carcinogenesis. |
| Document Type: Article |
| Language: English |
| Reprint Address: Faux, SP (reprint author), Univ Leicester, MRC, Toxicol Unit, Hodgkin Bldg, Leicester LE1 9HN, Leics England |
Addresses:
1. Univ Leicester, MRC, Toxicol Unit, Leicester LE1 9HN, Leics England
2. Univ Leicester, Ctr Mech Human Tox, Leicester LE1 9HN, Leics England |
| Publisher: OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND |
| Subject Category: Oncology |
| IDS Number: 391FZ |
| ISSN: 0143-3334 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |