| | |  | | | | Record from Web of Science® | | | | | | |
| Crystal structure of an initiation factor bound to the 30S ribosomal subunit |
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| Author(s): Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Hartsch T, Wimberly BT, Ramakrishnan V |
| Source: SCIENCE Volume: 291 Issue: 5503 Pages: 498-501 Published: JAN 19 2001 |
| Times Cited: 185 References: 40 |
| Abstract: Initiation of translation at the correct position on messenger RNA is essential for accurate protein synthesis. In prokaryotes, this process requires three initiation factors: IF1, IF2, and IF3, Here we report the crystal structure of a complex of IF1 and the 30S ribosomal subunit, Binding of IF1 occludes the ribosomal A site and flips out the functionally important bases A1492 and A1493 from helix 44 of 16S RNA, burying them in pockets in IF1. The binding of IF1 causes long-range changes in the conformation of H44 and Leads to movement of the domains of 30S with respect to each other. The structure explains how localized changes at the ribosomal A site lead to global alterations in the conformation of the 30S subunit. |
| Document Type: Article |
| Language: English |
| Reprint Address: Ramakrishnan, V (reprint author), MRC, Mol Biol Lab, Hills Rd, Cambridge CB2 2QH, England |
Addresses:
1. MRC, Mol Biol Lab, Cambridge CB2 2QH, England
2. Univ Gottingen, Inst Genet & Mikrobiol, Gottingen Genom Lab, D-37077 Gottingen, Germany |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 393UF |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |