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| Distinct CpG DNA and polyinosinic-polycytidylic acid double-stranded RNA, respectively, stimulate CD11c(-) type 2 dendritic cell precursors and CD11c(+) dendritic cells to produce type IIFN |
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| Author(s): Kadowaki N, Antonenko S, Liu YJ |
| Source: JOURNAL OF IMMUNOLOGY Volume: 166 Issue: 4 Pages: 2291-2295 Published: FEB 15 2001 |
| Times Cited: 171 References: 40 |
| Abstract: Two classes of nucleic acids, bacterial DNA containing unmethylated CPG motifs and dsRNA in viruses, induce the production of type I IFN that contributes to the immunostimulatory effects of these microbial molecules. Thus, it is important to determine which cells produce type I IFN in response to CpG DNA and dsRNA, CD4(+)CD11c(-) type 2 dendritic cell precursors (pre-DC2) were identified as the main producers of type I IFN in human blood in response to viruses. Here we asked whether pre-DC2 also produce type I HN in response to CpG DNA and dsRNA, Oligodeoxynucleotides containing particular palindromic CpG motifs induced pre-DC2, but not CD11c(+) blood DC or monocytes, to produce IFN-cu. In contrast, a synthetic dsRNA, polyinosinic polycytidylic-acid, induced CD11c(+) DC, but not pre-DC2 or monocytes, to produce IFN-alpha beta. These data indicate that CpG DNA and polyinosinic-polycytidylic acid stimulate different types of cells to produce type IFN and that it is important to select oligodeoxynucleotides containing particular CpG motifs to induce pre-DC2 to produce type I IFN, which may play a key role in the strong adjuvant effects of CpG DNA. |
| Document Type: Article |
| Language: English |
| Reprint Address: Kadowaki, N (reprint author), Kyoto Univ, Grad Sch Med, Dept Hematol & Oncol, Sakyo Ku, 54 Shogoin Kawara Cho, Kyoto 6068507, Japan |
Addresses:
1. DNAX Res Inst Mol & Cellular Biol Inc, Dept Immunobiol, Palo Alto, CA 94304 USA |
| Publisher: AMER ASSOC IMMUNOLOGISTS, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA |
| Subject Category: Immunology |
| IDS Number: 400QA |
| ISSN: 0022-1767 |
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