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| Expression and localization of epithelial aquaporins in the adult human lung |
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| Author(s): Kreda SM, Gynn MC, Fenstermacher DA, Boucher RC, Gabriel SE |
| Source: AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY Volume: 24 Issue: 3 Pages: 224-234 Published: MAR 2001 |
| Times Cited: 79 References: 31 |
| Abstract: Aquaporins (AQPs) facilitate water transport across epithelia and play an important role in normal physiology and disease in the human airways. We used in situ hybridization and immunofluorescence to determine the expression and cellular localization of AQPs 5, 4, and 3 in human airway sections. In nose and bronchial epithelia, AQP5 is expressed at the apical membrane of columnar cells of the superficial epithelium and submucosal gland acinar cells. AQP4 was detected in basolateral membranes in ciliated ducts and by in situ in gland acinar cells. AQP3 is present on basal cells of both superficial epithelium and gland acinus. In these regions AQPs 5, 4, and 3 are appropriately situated to permit transepithelial water permeability. In the small airways (proximal and terminal bronchioles) AQP3 distribution shifts from basal cell to surface expression (i.e., localized to the apical membrane of proximal and terminal bronchioles) and is the only AQP identified in this region of the human lung. The alveolar epithelium has all three AQPs represented, with AQP5 and AQP4 localized to type I pneumocytes and AQP3 to type II cells. This study describes an intricate network of AQP expression that mediates water transport across the human airway epithelium. |
| Document Type: Article |
| Language: English |
| Reprint Address: Gabriel, SE (reprint author), Univ N Carolina, Cyst Fibrosis Pulm Res & Clin Treatment Ctr, 6007 Thurston Bowles, Chapel Hill, NC 27599 USA |
Addresses:
1. Univ N Carolina, Cyst Fibrosis Pulm Res & Clin Treatment Ctr, Chapel Hill, NC 27599 USA |
| Publisher: AMER THORACIC SOC, 1740 BROADWAY, NEW YORK, NY 10019-4374 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology; Respiratory System |
| IDS Number: 416RY |
| ISSN: 1044-1549 |
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