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| Human-ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8 |
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| Author(s): Charlier C, Segers K, Wagenaar D, Karim L, Berghams S, Jaillon O, Shay T, Weissenbach J, Cockett N, Gyapay G, Georges M |
| Source: GENOME RESEARCH Volume: 11 Issue: 5 Pages: 850-862 Published: MAY 2001 |
| Times Cited: 88 References: 37 |
| Abstract: Two ovine BAC clones and a connecting long-range PCR product, jointly spanning similar to 250 kb and representing most of the MULGES-OY3 marker interval known to contain the ck locus, were completely sequenced. The resulting genomic sequence was aligned with its human ortholog and extensively annotated. Six transcripts, four of which were novel, were predicted to originate from within the analyzed region and their existence confirmed experimentally: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. RT-PCR experiments performed on a range of tissues sampled from an 8-wk-old animal demonstrated the preferential expression of all six transcripts in skeletal muscle, which suggests that they are under control of common regulatory elements. The six transcripts were also shown to be subject to parental imprinting: DLK1, DAT, and PEG11 were shown to be paternally expressed and GTL2, antiPEG11, and MEG8 to be maternally expressed. |
| Document Type: Article |
| Language: English |
| Reprint Address: Georges, M (reprint author), Univ Liege, Fac Vet Med, Dept Genet, B-4000 Liege, Belgium |
Addresses:
1. Univ Liege, Fac Vet Med, Dept Genet, B-4000 Liege, Belgium
2. Genoscope, Ctr Natl Sequencage, F-91057 Evry, France
3. Utah State Univ, Coll Agr, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: 429DW |
| ISSN: 1088-9051 |
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