| | |  | | | | Record from Web of Science® | | | | | | |
| Integrated genomic and proteomic analyses of a systematically perturbed metabolic network |
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| Author(s): Ideker T, Thorsson V, Ranish JA, Christmas R, Buhler J, Eng JK, Bumgarner R, Goodlett DR, Aebersold R, Hood L |
| Source: SCIENCE Volume: 292 Issue: 5518 Pages: 929-934 Published: MAY 4 2001 |
| Times Cited: 922 References: 29 |
| Abstract: We demonstrate an integrated approach to build, test, and refine a model of a cellular pathway, in which perturbations to critical pathway components are analyzed using DNA microarrays, quantitative proteomics, and databases of known physical interactions. Using this approach, we identify 997 messenger RNAs responding to 20 systematic perturbations of the yeast galactose-utilization pathway, provide evidence that approximately 15 of 289 detected proteins are regulated posttranscriptionally, and identify explicit physical interactions governing the cellular response to each perturbation. We refine the model through further iterations of perturbation and global measurements, suggesting hypotheses about the regulation of galactose utilization and physical interactions between this and a variety of other metabolic pathways. |
| Document Type: Article |
| Language: English |
| Reprint Address: Ideker, T (reprint author), Inst Syst Biol, 4225 Roosevelt Way NE,Suite 200, Seattle, WA 98105 USA |
Addresses:
1. Inst Syst Biol, Seattle, WA 98105 USA
2. Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
3. Univ Washington, Dept Comp Sci, Seattle, WA 98195 USA
4. Univ Washington, Dept Microbiol, Seattle, WA 98195 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 429KB |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |