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| Aberrations of chromosome 8 in 16 breast cancer cell lines by comparative genomic hybridization, fluorescence in situ hybridization, and spectral karyotyping |
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| Author(s): Rummukainen J, Kytola S, Karhu R, Farnebo F, Larsson C, Isola JJ |
| Source: CANCER GENETICS AND CYTOGENETICS Volume: 126 Issue: 1 Pages: 1-7 Published: APR 1 2001 |
| Times Cited: 26 References: 33 |
| Abstract: Comparative genomic hybridization (CGH) studies have shown that chromosome 8 is a frequent target for chromosomal aberrations in breast cancer. We characterized these aberrations of chromosome 8 in 16 breast cancer cell lines (BT-474, BT-549, CAMA-1, DU-4475, MCF-7, MDA-MB-134, MDA-MB-157, MDA-MB-361, MDA-MB-415, MDA-MB-436, MPE600, SK-BR-3, T-47D, UACC-812, UACC-893 and ZR-75-1) by CGH, fluorescence in situ hybridization (FISH) with arm- and locus-specific probes, and spectral karyotyping (SKY). Chromosome 8 was structurally abnormal in 13 of 16 cell lines. Loss of 8p was detected in nine cell lines, gain of entire Sq in six cell lines, 8q21-qter in three, 8q23-qter in two, and 8q12-qter and 8p21-q21 in one cell line. Extra copies of the C-MYC oncogene were found in 11 cell lines, but high-level amplification only in SK-BR-3. Derivative chromosomes including material from chromosomes 8 were complex, and the breakpoints were strikingly dissimilar. Chromosome 11 was the most frequent translocation partner with chromosome 8 (in 7 cell lines). Isochromosomes and/or isoderivative Sq were found in four cell lines. The high frequency and complexity of alterations at Sq indicate a significant pathogenetic role in breast cancer. The high-level amplification of c-myc is less common than previously thought. (C) 2001 Elsevier Science Inc. All rights reserved. |
| Document Type: Article |
| Language: English |
| Reprint Address: Rummukainen, J (reprint author), Tampere Univ, Inst Med Technol, Canc Genet Lab, POB 607, FIN-33101 Tampere, Finland |
Addresses:
1. Tampere Univ, Inst Med Technol, Canc Genet Lab, FIN-33101 Tampere, Finland
2. Tampere Univ Hosp, FIN-33101 Tampere, Finland
3. Karolinska Hosp, Dept Mol Med, S-10401 Stockholm, Sweden |
| Publisher: ELSEVIER SCIENCE INC, 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA |
| Subject Category: Oncology; Genetics & Heredity |
| IDS Number: 433CD |
| ISSN: 0165-4608 |
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