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| Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion |
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| Author(s): Becskei A, Seraphin B, Serrano L |
| Source: EMBO JOURNAL Volume: 20 Issue: 10 Pages: 2528-2535 Published: MAY 15 2001 |
| Times Cited: 242 References: 33 |
| Abstract: Feedback is a ubiquitous control mechanism of gene networks. Here, we have used positive feedback to construct a synthetic eukaryotic gene switch in Saccharomyces cerevisiae, Within this system, a continuous gradient of constitutively expressed transcriptional activator is translated into a cell phenotype switch when the activator is expressed autocatalytically. This finding is consistent with a mathematical model whose analysis shows that continuous input parameters are converted into a bimodal probability distribution by positive feedback, and that this resembles analog-digital conversion. The autocatalytic switch is a robust property in eukaryotic gene expression. Although the behavior of individual cells within a population is random, the proportion of the cell population displaying either low or high expression states can be regulated. These results have implications for understanding the graded and probabilistic mechanisms of enhancer action and cell differentiation. |
| Document Type: Article |
| Language: English |
| Reprint Address: Becskei, A (reprint author), European Mol Biol Lab, Meyerhofstr 1, D-69012 Heidelberg, Germany |
Addresses:
1. European Mol Biol Lab, D-69012 Heidelberg, Germany |
| Publisher: OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 436PC |
| ISSN: 0261-4189 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |