| Conditional gene knockout using Cre recombinase |
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| Author(s): Le YZ, Sauer B |
| Source: MOLECULAR BIOTECHNOLOGY Volume: 17 Issue: 3 Pages: 269-275 Published: MAR 2001 |
| Times Cited: 30 References: 19 |
| Abstract: Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of c rr gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein. |
| Document Type: Article |
| Language: English |
Addresses:
1. Oklahoma Med Res Fdn, Dev Biol Program, Oklahoma City, OK 73104 USA |
| Publisher: HUMANA PRESS INC, 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| IDS Number: 441PE |
| ISSN: 1073-6085 |