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| Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene |
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| Author(s): Bartee L, Malagnac F, Bender J |
| Source: GENES & DEVELOPMENT Volume: 15 Issue: 14 Pages: 1753-1758 Published: JUL 15 2001 |
| Times Cited: 148 References: 28 |
| Abstract: Plants maintain cytosine methylation at CG and non-CG residues to control gene expression and genome stability. In a screen for Arabidopsis mutants that alter methylation and silencing of a densely methylated endogenous reporter gene, we recovered 11 loss-of-function alleles in the CMT3 chromomethylase gene. The cmt3 mutants displayed enhanced expression and reduced methylation of the reporter, particularly at non-CC cytosines. CNG methylation was also reduced at repetitive centromeric sequences. Thus, CMT3 is a key determinant for non-CC methylation. The lack of CMT homologs in animal genomes could account for the observation that in contrast to plants, animals maintain primarily CG methylation. |
| Document Type: Article |
| Language: English |
| Reprint Address: Bender, J (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Biochem & Mol Biol, Baltimore, MD 21205 USA |
Addresses:
1. Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Biochem & Mol Biol, Baltimore, MD 21205 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: 455GW |
| ISSN: 0890-9369 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |